Here are some time lapse movies of various cell types growing in 3D culture on top of Matrigel.

Clicking on the images below will load a video file in .avi format. Each file is approximately 10 MB.

T4-2 breast cancer cells. These cells form large tumor-like colonies in 3D culture.

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T4-2 breast cancer cells treated with a small molecule inhibitor of EGFR (AG1478). These cells form small, smooth polarized growth-arrested colonies similar to non-malignant mammary acini.

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SVEC4-10 mouse endothelial cells. These cells quickly form tubules in 3D culture.